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玫瑰花总黄酮对乳腺增生大鼠上皮-间质转换的影响
Effects of total flavonoids from Rosa rugosa on epithelial-to-mesenchymal transition in rats with hyperplasia of mammary gland
目的： 探讨玫瑰花总黄酮对实验性乳腺增生大鼠上皮-间质转换（EMT）的影响。方法： 通过注射雌激素和孕激素诱导大鼠乳腺增生，观察玫瑰花总黄酮（200和100 mg·kg-1·d-1）给药干预对乳腺组织形态学变化、乳腺fibronectin、α-SMA、E-cadherin、collagen-I和TGF-β1表达情况，以及微小RNA（miR）-21和miR-29a/b水平的影响。结果： 玫瑰花总黄酮给药能显著改善模型动物乳腺增生的趋势，抑制乳腺组织collagen-I沉积和TGF-β1表达，缓解EMT上皮标记物E-cadherin的缺失以及间质标记物fibronectin和α-SMA等的过度表达，降低miR-21并一定程度恢复miR-29a/b的水平。结论： 玫瑰花总黄酮对乳腺EMT发生的程度具有潜在的抑制作用。
OBJECTIVE To assess effects of total flavonoids from Rosa rugosa (FRR) on epithelial-to-mesenchymal transition (EMT) in rats with hyperplasia of mammary gland (HMG).METHODS Rat model of HMG was induced by intramuscular injection of estrogen and progestogen.Effects of FRR (200 or 100 mg·kg-1·d-1) on mammary morphology changes,expressions of fibronectin,α-SMA,E-cadherin,collagen-I and TGF-β1,as well as levels of microRNA (miR)-21 and miR-29a/b were investigated.RESULTS FRR obviously approved mammary morphology disorders,decreased collagen-I and TGF-β1 expression,and inhibited deletion of EMT characteristic epithelial marker E-cadherin,and over-expression of mesenchymal markers fibronectin and α-SMA.FRR also reduced mammary level of miR-21 and enhanced level of miR-29a/b.CONCLUSION FRR is able to inhibit EMT in rats.
玫瑰花 / 乳腺增生 / 上皮-间质转换 {{custom_keyword}} /
Rosa rugosa / hyperplasia of mammary gland / epithelial-to-mesenchymal transition {{custom_keyword}} /
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湖北省自然科学基金（编号：2013CFB361）
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