玫瑰花总黄酮对乳腺增生大鼠上皮-间质转换的影响

吕操, 杜丽芬, 宋红萍

中国医院药学杂志 ›› 2016, Vol. 36 ›› Issue (16) : 1362-1365.

PDF(1321 KB)
PDF(1321 KB)
中国医院药学杂志 ›› 2016, Vol. 36 ›› Issue (16) : 1362-1365. DOI: 10.13286/j.cnki.chinhosppharmacyj.2016.16.07
研究论文

玫瑰花总黄酮对乳腺增生大鼠上皮-间质转换的影响

  • 吕操, 杜丽芬, 宋红萍
作者信息 +

Effects of total flavonoids from Rosa rugosa on epithelial-to-mesenchymal transition in rats with hyperplasia of mammary gland

  • LYU Cao, DU Li-fen, SONG Hong-ping
Author information +
文章历史 +

摘要

目的: 探讨玫瑰花总黄酮对实验性乳腺增生大鼠上皮-间质转换(EMT)的影响。方法: 通过注射雌激素和孕激素诱导大鼠乳腺增生,观察玫瑰花总黄酮(200和100 mg·kg-1·d-1)给药干预对乳腺组织形态学变化、乳腺fibronectin、α-SMA、E-cadherin、collagen-I和TGF-β1表达情况,以及微小RNA(miR)-21和miR-29a/b水平的影响。结果: 玫瑰花总黄酮给药能显著改善模型动物乳腺增生的趋势,抑制乳腺组织collagen-I沉积和TGF-β1表达,缓解EMT上皮标记物E-cadherin的缺失以及间质标记物fibronectin和α-SMA等的过度表达,降低miR-21并一定程度恢复miR-29a/b的水平。结论: 玫瑰花总黄酮对乳腺EMT发生的程度具有潜在的抑制作用。

Abstract

OBJECTIVE To assess effects of total flavonoids from Rosa rugosa (FRR) on epithelial-to-mesenchymal transition (EMT) in rats with hyperplasia of mammary gland (HMG).METHODS Rat model of HMG was induced by intramuscular injection of estrogen and progestogen.Effects of FRR (200 or 100 mg·kg-1·d-1) on mammary morphology changes,expressions of fibronectin,α-SMA,E-cadherin,collagen-I and TGF-β1,as well as levels of microRNA (miR)-21 and miR-29a/b were investigated.RESULTS FRR obviously approved mammary morphology disorders,decreased collagen-I and TGF-β1 expression,and inhibited deletion of EMT characteristic epithelial marker E-cadherin,and over-expression of mesenchymal markers fibronectin and α-SMA.FRR also reduced mammary level of miR-21 and enhanced level of miR-29a/b.CONCLUSION FRR is able to inhibit EMT in rats.

关键词

玫瑰花 / 乳腺增生 / 上皮-间质转换

Key words

Rosa rugosa / hyperplasia of mammary gland / epithelial-to-mesenchymal transition

引用本文

导出引用
吕操, 杜丽芬, 宋红萍. 玫瑰花总黄酮对乳腺增生大鼠上皮-间质转换的影响[J]. 中国医院药学杂志, 2016, 36(16): 1362-1365 https://doi.org/10.13286/j.cnki.chinhosppharmacyj.2016.16.07
LYU Cao, DU Li-fen, SONG Hong-ping. Effects of total flavonoids from Rosa rugosa on epithelial-to-mesenchymal transition in rats with hyperplasia of mammary gland[J]. Chinese Journal of Hospital Pharmacy, 2016, 36(16): 1362-1365 https://doi.org/10.13286/j.cnki.chinhosppharmacyj.2016.16.07
中图分类号: R285.5   

参考文献

[1] 中国药典.一部[S].2015:200.
[2] 国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科技出版社,1999:238-241.
[3] 陈涛,宋红萍,陈镜楼.玫瑰花总黄酮纯化工艺和口服毒性研究[J].时珍国医国药,2014,25(9):2105-2107.
[4] 张亚迎,陈涛,宋红萍.玫瑰花对脂多糖诱导的大鼠乳腺炎的影响[J].中国药师,2016,19(1):33-35.
[5] Chen T,Li J,Chen J,et al.Anti-hyperplasia effects of Rosa rugosa polyphenols in rats with hyperplasia of mammary gland[J].Environ Toxicol Pharmacol,2015,39(2):990-996.
[6] 刘凯,许惠琴,陆春红,等.精制消癥丸调控乳腺增生大鼠性激素及其受体表达[J].中国医院药学杂志,2015,35(1):15-19.
[7] Das Gupta S,So JY,Wall B,et al.Tocopherols inhibit oxidative and nitrosative stress in estrogen-induced early mammary hyperplasia in ACI rats[J].Mol Carcinog,2015,54(9):916-925.
[8] 丁旭娜.TGF-β1、bFGF和PDGF在奶牛乳腺纤维化中作用的研究[D].呼和浩特:内蒙古农业大学,2012.
[9] Liu X,Yun F,Shi L,et al.Roles of signaling pathways in the Epithelial-Mesenchymal transition in cancer[J].Asian Pac J Cancer Prev,2015,16(15):6201-6206.
[10] Gonzalez DM,Medici D.Signaling mechanisms of the epithelial-mesenchymal transtition[J].Sci Signal,2014,7(344):re8.
[11] 王智森,高飞,陆付耳.红景天提取物对肾小球系膜细胞株增殖及分泌细胞外基质的影响[J].中国医院药学杂志,2014,34(11):863-867.
[12] 赵娟,曾欣,林勇.微小RNA调控器官纤维化的研究进展[J].肝脏,2012,17(6):423-425.
[13] Sandhu GK,Milevskiy MJ,Wilson W,et al.Non-coding RNAs in Mammary Gland Development and Disease[J].Adv Exp Med Biol,2016,886:121-153.
[14] Sturrock A,Mir-Kasimov M,Baker J,et al.Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress[J].J Biol Chem,2014,289(7):4095-4105.
[15] An HJ,Kim IT,Park HJ,et al.Tormentic acid,a triterpenoid saponin,isolated from Rosa rugosa,inhibited LPS-induced iNOS,COX-2,and TNF-α expression through inactivation of the nuclear factor-κb pathway in RAW 264.7 macrophages[J].Int Immunopharmacol,2011,11(4):504-510.

基金

湖北省自然科学基金(编号:2013CFB361)

PDF(1321 KB)

Accesses

Citation

Detail

段落导航
相关文章

/